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                  AgPath-ID™ One-Step RT-PCR Reagents

                  描述

                  AgPath-ID™ 一步法RT-PCR试剂盒利用一种快速单管实时荧光定量逆转录PCR方法ฃฌ可灵敏稳定地扩增RNA靶点กฃ

                  • 推荐用于RNA病原体扩增
                  • 一致的RNA靶位扩增ฃฌ高特异性ฃฌ高灵敏度
                  • 已经过优化ฃฌ与靶位特异性引物和探针一同使用
                  • 含有ROXฃฌ可用于定量荧光信号的标准化

                  AgPath-ID™ AgPath-ID™ 一步法 RT-PCR试剂配置用于快速简单地设置反应กฃ所有反应集于一管ฃฌ最大程度降低样本处理错误ฃฌ缩短设置时间กฃ组装好之后ฃฌ约1小时内即可获得结果กฃ试剂盒中的RT-PCR酶混合液组分有高效的 ArrayScript™ 逆转录酶กข可提供高产量cDNA的突变MMLV逆转录酶和用于特异性靶点扩增的热启动DNA聚合酶—AmpliTaq Gold® 聚合酶กฃ2X RT-PCR缓冲液包含有优化的试剂ฃฌ用于高效稳定地进行的逆转录和PCRฃป缓冲液中还含有专利参比荧光ROXฃฌ用于定量荧光信号的标准化กฃ该试剂盒还提供了可选的检测增强剂ฃฌ可扩增具有富GC区或顽固二级结构的模板กฃ
                  For Research Use Only. Not for use in diagnostic procedures.

                  规格

                  PCR Method: 1-Step qRT-PCR
                  Reverse Transcriptase: ArrayScript™ Reverse Transcriptase
                  Polymerase: AmpliTaq Gold® DNA Polymerase
                  Detection Method: Primer-Probe Detection
                  Primer-Probe Compatibility: TaqMan®
                  Reaction Speed: Standard
                  Platform: 7500 System
                  Sample Type (General): RNA
                  Passive Reference Dye: ROX (Pre-mixed)
                  Comments: Works on all AB platforms, but is optimized for the 7500.
                  Product Size: 100 reactions
                  Shipping Condition: Dry Ice

                  内容及储存

                  Sufficient for 100 reactions of 25 µL, contains:
                  • 1375µL 2X RT-PCR Buffer
                  • 110µL 25X RT-PCR Enzyme Mix
                  • 190µL Detection Enhancer
                  • 1.75mL Nuclease-free Water.

                  上一篇ฃบMagMAX™-96 Viral RNA Isolation Kit 下一篇?#22909;?#26377;了


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