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                  AgPath-ID™ One-Step RT-PCR Reagents

                  描述

                  AgPath-ID™ 一步法RT-PCR試劑盒利用一種快速單管實時熒光定量逆轉錄PCR方法,可靈敏穩定地擴增RNA靶點。

                  • 推薦用于RNA病原體擴增
                  • 一致的RNA靶位擴增,高特異性,高靈敏度
                  • 已經過優化,與靶位特異性引物和探針一同使用
                  • 含有ROX,可用于定量熒光信號的標準化

                  AgPath-ID™ AgPath-ID™ 一步法 RT-PCR試劑配置用于快速簡單地設置反應。所有反應集于一管,最大程度降低樣本處理錯誤,縮短設置時間。組裝好之后,約1小時內即可獲得結果。試劑盒中的RT-PCR酶混合液組分有高效的 ArrayScript™ 逆轉錄酶、可提供高產量cDNA的突變MMLV逆轉錄酶和用于特異性靶點擴增的熱啟動DNA聚合酶—AmpliTaq Gold® 聚合酶。2X RT-PCR緩沖液包含有優化的試劑,用于高效穩定地進行的逆轉錄和PCR;緩沖液中還含有專利參比熒光ROX,用于定量熒光信號的標準化。該試劑盒還提供了可選的檢測增強劑,可擴增具有富GC區或頑固二級結構的模板。
                  For Research Use Only. Not for use in diagnostic procedures.

                  規格

                  PCR Method: 1-Step qRT-PCR
                  Reverse Transcriptase: ArrayScript™ Reverse Transcriptase
                  Polymerase: AmpliTaq Gold® DNA Polymerase
                  Detection Method: Primer-Probe Detection
                  Primer-Probe Compatibility: TaqMan®
                  Reaction Speed: Standard
                  Platform: 7500 System
                  Sample Type (General): RNA
                  Passive Reference Dye: ROX (Pre-mixed)
                  Comments: Works on all AB platforms, but is optimized for the 7500.
                  Product Size: 100 reactions
                  Shipping Condition: Dry Ice

                  內容及儲存

                  Sufficient for 100 reactions of 25 µL, contains:
                  • 1375µL 2X RT-PCR Buffer
                  • 110µL 25X RT-PCR Enzyme Mix
                  • 190µL Detection Enhancer
                  • 1.75mL Nuclease-free Water.

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